You might be probably applied to obtaining internet sites by going to a search engine which include Google or Bing. In case you are a lengthy time web user you may keep in mind other search engines including Lycos and Alta Vista. When you get there, you sort some parameters into the search box and hope for the very best. With some topics you?ll uncover best internet sites this way, with others you may not. The cause is that search engine ranking is carried out by machine having a really complicated algorithm. Webmasters who have an understanding of Seo (search engine optimization) and who can afford to pay for back link building services can often get their sites ranked way above the ideal web-sites which have extra fascinating content.
Years ago you can go to yahoo.com and browse by means of their lists of web pages. These sites were manually reviewed by actual human beings, not computers. Those days are lengthy gone.
Nonetheless there is certainly nonetheless a method to obtain web pages that have been suggested by humans, not by computers. In case you have a Facebook page, you can occasionally come across a recommendation for an interesting internet web-site from one of one?s buddies, or at least from 1 of their buddies. Because you realize these people, you may make an intelligent judgment about no matter if to invest your time viewing the web site they?ve recommended.
You could extend this principle to search much more social media websites, for instance Digg or LinkedIn to find internet sites that may well interest you. There are numerous far more social media web pages on the web than many people understand. Attempt some of the bigger, a lot more nicely known ones very first. A lot of of them will enable you to either search for the kind of content you are trying to find or to set up an RSS feed to send the recommendations proper to your pc automatically.
Mashtop presents an unique way to come across and share the top sites on line, curated by an professional community of social media gurus.
What's New: Fans of the restyled 5 Series were up in arms over its heavier, more luxurious underpinnings. So there was plenty to potentially bungle with the all-new M5. But BMW went to great lengths to ensure that its flagship's sporting sanctity will satisfy rabid enthusiasts. Though still 90 pounds heavier than a comparably equipped 550i, the new M5 got lighter through aluminum components and by ditching the huge axle bushings. Now that the rear suspension is bolted directly to the chassis, this two-ton ride feels more in tune with the road. A hydraulic steering setup also replaces the standard 5 Series' electric arrangement and has a crisper feel. Cockpit-adjustable shocks round out a tightly tuned suspension package, and heavier-duty six-piston brakes assure strong stops with the 18- or 19-inch wheels.
BMW once proclaimed that M cars would never be turbocharged. Times have changed: This M5's bulged hood hides a twin scroll, twin-turbocharged V8 adapted from the X5 M and X6 M. The mill churns 560 hp and 500 lb-ft of torque. The direct-injected powerplant achieves peak torque between 1500 and 5750 rpm and claims 30 percent better fuel economy than its predecessor, thanks in part to an automatic Stop/Start feature (which can be disabled). Replacing the last generation's SMG automated manual is an M3-sourced seven-speed dual clutch unit that's been reinforced and outfitted with taller ratios.
Tech Tidbit: Although BMW engineers say the M5's twin-turbocharged V8 is "at the edge" of global noise limits, the insulated cabin remains rather isolated from the car's growling exhaust note. But if you're driving an M5, you want it to sound like an M5. So the Bavarian automaker devised a clever solution: It incorporated a digital signal processor that translates data from the engine management system and reproduces engine noises through the car's six-speaker audio system. Voil?! A virtual sport exhaust that won't rattle the neighbors.
Driving Character: The M5's leather and aluminum-trimmed interior is more purposeful than opulent. But the mellow, nearly subliminal exhaust hum from the turbocharged V8 powerplant suggests silky smooth refinement, which this big sedan exudes when driven calmly. Leave the throttle, steering, suspension and shift modes in "Comfort," and you might be hard-pressed to believe this is an "M" car. But switch it up and mash the throttle, and this understated sedan transforms into an angry four-door supercar.
The twisting highways just south of Seville, Spain, make the 560-hp engine seem a bit excessive for public roads?all that power sporadically kicks out the tail end, and triple-digit speeds arrive faster than you can say, "Lo siento, polic?a!" So we breathed a sigh of relief upon arriving at Ascari Race Resort, where 3.37 miles and 26 turns awaited our Monte Carlo Blue metallic monster. Even with a little morning rain, the M5's Michelin Pilot Super Sports dug into the track's well-kept surfaces. We nearly forget we were driving more than 4000 pounds of sedan. But what really took us by surprise was how maneuverable the new M5 proved to be, despite its longer wheelbase. Credit the ultraflexible engine, seven cogs to choose from, and a buttoned-down chassis that seems unflappable when pushed near the limit. An active rear differential apportions power to the side that needs it the most, and the hydraulic steering system conveys enough information to inspire confidence.
When the kinks straighten out, the engine power pushes the M5 forward under breathtaking g-forces. The dual clutch transmission's shifts were lightning fast yet surprisingly smooth, enabling mid-corner cog swaps that didn't upset the car's balance. And finally, the M5's six-piston brakes performed as advertised, though we wouldn't be surprised if would-be racers choose the pricey carbon-ceramic option if and when it becomes available in the U.S.
Favorite Detail: Compared with its predecessors, the newest M5 is a more understated sedan with a quiet cabin yet stunning performance?a virtual textbook example of what constitutes a compelling Q-ship.
Driver's Grievance: Maybe it's too refined. What makes this ultra-high-performance sedan so great also detracts from its desirability. There's no arguing its extreme capabilities (which we confirmed during hot laps), but the new M5 begs for more edge and a touch more spine-tingling personality.
The Bottom Line: While even die-hard drivers criticized the last generation's jerky automated manual gearbox and 8250 rpm Formula One?derived V10, this M car offers blistering performance along with enough electronic controls to switch it from track day beast into a compliant daily driver. Boy-racer types may wish for obvious symbols of outward speed, such as a burlier exhaust note or more aggressive bodywork. But for those who savor the subtlety of an understated autobahn stormer, it doesn't get much more sublime than the 2013 BMW M5.
It has been 1 year and 2 months since D-Day and my husband and I have been working very hard to put our marriage back together. We went to counseling for about 3 months but had to discontinue it due to finances. However, we have read about a dozen books and have had endless hours of converstation. Thankfully, we are doing pretty well all considering.
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However, my problem is with my attitude toward the other woman. I saw her the other day and it just left me feeling angry and hurt all over again. I want so desperately to forgive her and be able to lay all these feelings to rest. But all it takes is for me to see her again and I feel like a little girl and so vulnerable. I hate that she has this affect on me.
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I pray for her almost daily. I pray for her marriage and I also pray that she get a revelation of how much pain she has caused to people that didn't deserve it. Right before seeing her, I actually found myself feeling less angry toward her and more gracious. Then when I saw her, all of that just walked out the door.
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I know that I struggle with the fact that she intentionally set out to try to take my husband from me. It wasn't a slow, gradual emotional connection and then it just happened. She set her sights on him and had the sole intention of getting him for herself and trying to have a life with him. She is unhappily married and she wanted someone to take her away from that relationship. I can forgive things that are not intentional. It's the intentional that is really throwing me.
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I know the Bible says,"forgive them for they know not what they do". I try to keep focused on that. But it's not helping. Would someone plrease give me some guidance on this? I really don't want this woman having this kind of control over me anymore. I know that my unforgiveness is only hurting me and I want it to stop.
Posted by Jake on September 27, 2011 Leave a Comment ?
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Monetary freedom is only able to be acknowledged with tough function, innovation, along with a well known - but reliable - item or service. But with the World wide web small business, these three normally get cloudy with getting pretty much as well a lot of possibilities. It is possible to very easily get frustrated if researching the mind-boggling number of small business venues which are offered on the net. Although with e-books, a lot of tiny small business proprietors are finding economic freedom although offering long-lasting merchandise of value.
E-books are electronic guides that could contain visuals, pictures, audio, as well as content referring to any topic. They can also contain hyperlinks to numerous internet pages for special offers or maybe illustrations. It's very affordable to develop an e-book, however folks enjoy studying them every single day. E-books are well known on the net due to the fact they're typically affordable or cost-free and offer you advice and suggestions on a number of topics. The topics can be anything from "how to generate capital online" to "how to start a household small business." E-books could also cover far more certain topics like "how to make capital having a crafts business" or "cross-stitching suggestions for that newbie." The probabilities for e-book topics are unlimited.
E-book Property Business enterprise Details
Starting an e-book small business indicates you're offering e-books for sale on the net to some certain target audience. You may provide only 1 or a few e-books, or you might even provide hundreds of them. The option is yours, but certainly, the far more e-books you offer you, the far more chances you will have to generate a profitable revenue.
Here's how it performs. You will promote your e-books employing numerous approaches like an e-mail newsletter (e-zine), research engine campaigns, compensated advertisements, along with other techniques. Consumers will go to your site to study your presentation. When they obtain 1 of your e-books, they are going to obtain it immediately out of your site or from an e-mail. It is possible to even systemize the whole method to make factors less difficult on you and also the consumer.
The cause e-books are quite profitable for gaining economic freedom is due to the fact when they're designed, you don't have to do anything besides promote them. It is possible to develop an automated cash system that performs 24 hours each day, 7 days a week. E-books can be sold as well as downloadable by the customers although you sleep!
Resell E-books to Bring in Income
When you don't would like to create your own e-books, you can also develop a cash system and enjoy economic freedom by reselling e-books that other people have authored. With this type of household small business, you may obtain rights to numerous e-books, at times hundreds of them, and resell them as your own merchandise. Keep in thoughts that other businesses will likely be selling these titles as well. But the world wide web is so significant, there's lots of space for every person!
A number of businesses provide small business possibilities in which you will receive quite a few positive aspects together with hundreds of e-books to resell. These possibilities could cost a bit in advance, but are absolutely worth the investment for starting your own household small business. The e-book merchandise alone can easily generate a really healthy cash system for a long time to come.
As with any household small business, selling e-books will take some time to construct and promote. However when the ball's moving, you will enjoy economic freedom and success like in no way before!
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[unable to retrieve full-text content]Once the initial excitement of starting your own personal business has distressed off, it is quite easy indeed providing procrastination to seep inside your...
Filmmaker and scriptwriter Rakeysh Omprakash Mehra of "Rang De Basanti" fame says the history of Indian cinema shows villains were the greatest mirrors of the country's changing socio-political scene.
"The first villains were the British - they were villains (literally). Then the feudal lords became the villains in the rich-poor conflicts and after that your father became the villain (because he told you which room in the hostel you had to stay and whom you should wed). And then the system became the villain," Mehra said.
Mehra was in conversation with film-writer Jai Arjun Singh about "New Wave Cinema" at the Lit for Life festival presented by The Hindu in the capital Sunday.
The discussion was sponsored by Om Books International, which is publishing Mehra's film "Rang De Basanti" as a novel.
"Then came young India... But a new wave is generally something which washes everything away and leaves behind a fragrance. New wave was discovered in French cinema. People started relating to it and there was participation of audience in cinema -- movies became interactive," Mehra said.
He feels movies also reflected the mood in the country.
"If you look at Indian cinema - Hindi and South Indian cinema, it reflects the mood of the country in the last 60 years. (Former prime minister Jawaharlal) Nehru's call to build the nation (with socio-economic growth) from cycles to railway engines was echoed by the cinema.
"Soon the idea of independence started fading away. Bengal and Maharashtra were ravaged by famines and there was the China war. It gave us (images of) poverty, the ordinary man's fight for survival. The man would return home at the end of the day with a handful of grain. He didn't want to go to the theatre to watch his life - and so we came up with escapism," Mehra said.
The dancing hero - Shammi Kapoor -- appeared on screen and they (heroes) are still dancing, the filmmaker added.
"We got the boy-meets-girl, the villain who catches the boy, bashes him up and in the midst of it, they manage to sing 12 songs," Mehra said running the audience through the journey of Indian cinema.
It was followed by a spell of anger - that era was dominated by Bollywood superstar Amitabh Bachchan, who emerged as a voice of suppressed class and rose in the film firmament as the Angry Young Man.
"The whole thing about governance, politics and anarchy (the various political movements) gave birth to the anti-establishment hero," Mehra said.
Mehra, who grew up in the old Delhi area of the capital, has also written and directed two other movies, "Aks" (2001) starring Amitabh and "Delhi 6" starring Abhishek Bachchan.
Now the filmmaker harbours epic dreams.
"I want to adapt the story of Karan from Mahabharata - the episode in which his mother says she is not his mother," Mehra said.
"I want to do a full-blown war movie between India and China too," he added.
Anyone who walks into a tattoo parlor might be greeted with a wide selection of eye-catching and colorful tattoo designs, including all the pieces from simple to pictures which can be very complex. Except people who have already chosen their most popular picture, it might be very tough to choose out from the numerous tattoo designs on the spot. Even in case you have already chosen a design, the artist might have the option to current you with a quantity of different options to make your tattoo designs much more personable.
Before arriving for the application of a tattoo, it?s best to have a collection of tattoo designs in mind earlier than you enter the parlor. Although there are some exceptions, most women and men will naturally be drawn to different tattoo designs. Men, as an example, would possible choose one thing masculine, while women typically want one thing dainty and sexy. Of all tattoo designs, ladies generally select hearts, butterflies, flowers, lettering, etc. The most popular placement, for ladies, is the shoulder, lower again and ankle. Males, then again, typically favor that their tattoo designs be positioned on the upper arm, chest or back.
Whereas there are tattoo designs which may be gender specific, you will want to remember the actual fact that getting a tattoo is a really private factor and the image that is finally chosen needs to be one which displays something that is essential to the wearer. Upon discussing the assorted tattoo designs with the artist, the customer will gain a greater understanding of the different options and designs available.
Many people choose tattoo designs from these introduced on the parlor, while others choose to perform a little analysis and assist be sure that their design is not like any other. For sure tattoo designs, some people favor to look at real life photos or flick thru artwork for concepts pertaining to their tattoo designs. When you find a picture that you like, merely take it to the artist for an analysis as to whether or not the image might be recreated within the form of a tattoo.
This text is to be used for informational functions only. The information contained herein is simply not meant to be used instead of, or along with, skilled medical advice or recommendations for tattoo designs or placement. Previous to getting a tattoo, individuals ought to consult with their physician to ensure that it?s a protected process for them to endure. Some people mustn?t get a tattoo if they have certain medical situations or a weakened immune system. For further info, consult a physician or dermatologist.
Tribal and Decrease Again Tattoos
Tribal and Decrease Again Tattoos are extremely standard and can add a definite ?je ne sais quoi? to a person?s character. The real great factor about tribal and lower again tattoos is that they do not must be loud and obnoxious ineffective you want them to be, but relatively refined and stylish. It is a fashion that sometimes capitalizes on solid black lines and coloring (with some exceptions in fact) and it is usually composed of pointed and curved elements. It?s bold and eye catching. In short, it is a pretty wicked looking style!
Dragon Tattoos
Dragon tattoos, when finished by a talented artist can be a dynamic piece of art. The dragon is a classic tattoo motif, in style with men and women, and exhibits the profound influence of Japanese and Chinese tradition in Western tattooing. Dragons were round long before man set his footprint in the sands of time.
Religious Tattoos
Spiritual Tattoos like crosses, symbols and images of Christ are extremely fashionable and can add a distinct ?je ne sais quoi? to an individual?s character. The real magnificence of spiritual tattoos is that they do not should be loud and obnoxious useless you want them to be, however rather refined and stylish.
Chinese language and Kanji Tattoos Chinese Tattoos like chinese language calligraphy and kanji symbols are extraordinarily standard and can add a distinct ?je ne sais quoi? to an individual?s character. The real beauty of chinese language tattoos is that they do not need to be loud and obnoxious ineffective you want them to be, however somewhat refined and stylish.
Star Tattoos
Star tattoos, when achieved by a talented artist generally is a dynamic piece of art. Stars are often encountered as symbols, and lots of instances the that means of a selected star image may depend on the quantity points it has, and sometimes the orientation of those points as well.
The problem with on-line tattoo designs is that they can be pricey and sometimes very mundane. The answer is to seek out low price tattoo designs that are of good quality. You can do this by viewing the sample designs provided on the website.
For extra info and some glorious Tattoo Designs at very cheap prices I suggest you go to TattooSleeves.us The Finest On line Tattoo Designs that you can discover
Car has turn a key need of every adult particular to make easy travelling problems. Numbers of lenders are ready to offer automobile loans for bad credit and the contest of theirs? has done it easy and profitable to have a automobile loan. Moreover, it does not matter either you have a great credit, bad credit, no credit, accessibility of cosigner, or filed a bankruptcy.
Planning is really critical to fissure a auspicious deal. If you wish to purchase a automobile whilst having a low fascination used vehicle loans, you should initial examine out the merits and demerits of the loan. This investigate will help you since eventually you have to pay the monthly installments and upgrade your credit score.
Obtaining automobile loans with bad credit is an easy task. People with no credit normally includes students as they are still studying and do not have any chance to consequence credits. These variety of loans are feel safe loans and thus availing this will not be a tough charge even even though there is no credit. The lending hospital has no worries in box of default as they have the control of confiscating the car.
To relief automobile loan for bad or bad credit is moreover easy. The lending institutes can rest positive that they can grab the automobile in box of default concerning field with bad or bad credit. Therefore, they do not pause in giving bad credit automobile finance. However, the fascination rates are partially high.
If you are a student, then you can obtain a tyro automobile loan without cosigner usually if the loan amount does not exceeds the amount of $ 25,000. A cosigner is compulsory if the loan amount is aloft than this amount.
If you have filed a failure in past and right away you wish to purchase a car, the initial step of action is that you should examine your credit report. You should be particular in this matter of traffic a failure automobile loans. You should deliberate usually gifted lenders who specialize in failure and on trial automobile financing.
Playlists are a great way to organize your favorite music so that you're only listening to the tracks you want to. With iTunes it's easy to set these lists up. It's only a mater of dragging and dropping the songs or albums you want into your playlists; we'll show you step by step in our video.
For more episodes of Just Show Me, subscribe to Tecca TV's You Tube channel and check out all our Just Show Me episodes. If you have any topics you'd like to see us cover, just drop us a line in the comments.
Image by stateofplace The International athletics Committee has asked the concern governance embody for athletics to analyse whether members of the Asiatic women?s aggroup were likewise teen to contend in the Olympics.
The I.O.C. taught the planetary athletics federation, famous as the F.I.G., to verify up the supply with the Asiatic athletics federation and inform backwards to the I.O.C. after Friday.
The New royalty Times reportable terminal period that online records organisation Asiatic gymnasts and their ages that were posted on authorised Web sites in China, along with ages presented in the authorised Asiatic programme media, indicated that members of the aggroup strength be as teen as 14. A gymnast staleness invoke 16 in the assemblage of the athletics to contend in the Games. Asiatic officials responded by providing copies of passports indicating that the athletes in discourse were older enough.
?More aggregation has been brought to reddened and brought to our attention, so we definite to go to the federation and hit them countenance into it further,? said Emmanuelle Moreau, a spokeswoman for the I.O.C. ?We had been presented whatever more aggregation and intellection that this aggregation was concerning sufficiency to go to the Asiatic gymnastic federation and hit a complete communicating most it.?
According to online sports entrance lists in China, half the aggroup ? He Kexin, Yang Yilin, Jiang Yuyuan ? would be low age. The F.I.G., however, has said that those gymnasts were suitable and that the ages on their passports were correct.
The Asiatic women won the aggroup metallic among sextet amount medals at the Peiping Games. He won the metallic in the crinkly bars.
Moreau declined to feature what newborn aggregation had led to this stylish advise by the I.O.C., threesome life after the athletics rivalry ended. ?NYT 8/22/08
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This write-up sheds some light on "The VoIP phone system and our revolutionized communication technology". Even though nearly all of the time this matter has not been handed down it's proper segment in the media however now masses are communicating about VOIP and sharing knowledge concerning VOIP. Acquire thorough advantage of invaluable information in this article and please take time to comprehend it completely instead of just skimming through it.
The writer Abraham David is responsible for producing this article If you would like to take this article please link back to this web page. Article on "The VoIP phone system and our revolutionized communication technology" starts after this.
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The continuous revolution of technology is steadfastly converting our world into a better, more comfortable place to live in. Before, people only communicate through mails delivered by hand from one place to another. Years of innovation paved way to the use of telephones, beepers, and cellular phones. Communication technology?s significant breakthroughs are still spewing out from the brains of the experts, and so today, talking to anybody anywhere around the world is made cheaper with the use of a computer and an internet connection.
Voice over Internet Protocol (VoIP) is the latest technology allowing you to deliver and receive voice calls using an Internet connection, particularly broadband, as a substitute to an analog or an ordinary phone line. Also, it is referred to as the Broadband telephony, Voice over Broadband, IP Telephony Broadband Phone, and Internet telephony.
Your voice is translated into a digital signal that travels over the Internet through the VoIP services. Once calling a regular telephone number, the signal is coverts to a regular telephone signal just before it arrives at the destination. VoIP can let you make a call either directly from a special VoIP phone, a computer, or a traditional phone linked to one special adapter. Wireless ?hot spots? such as airports and parks allow you to connect to the Internet enabling you to use VoIP service wirelessly.
Ccomplex modern VoIP phone systems are made of several interlinked components. For most businesses, it is as complex and ambitious as they desire to let every employee have a phone with an extension. Furthermore, it requires voicemail for transferring calls and so forth. Unfortunately, this is not the way VoIP companies provide phone service. Modern business phone system is complicated and difficult as it is made up of three components or more.
The main components for having a VoIP phone system are the phones, the service, and the connection. The phones are the real units you hold for talking. The service refers to the company that makes the whole system work and connects phones to the universal telephone network. The connection are the wires or servers, gateways, switches, and routers that are running full tilt and connected to the Internet and to the PSTN.
Notice: Please beware that everything said in this composition on "The VoIP phone system and our revolutionized communication technology" ought to not be taken as suggestion from specialist, you should find out facts from more authentic places as well. Please ask a specialist of the applicable field. OvalEgg.com is not responsible for any damages of any kind that may arise from applying information contained in this article.
A broadband or a high speed Internet connection is one main requirement for having VoIP. Such connection can be managed with a cable modem or high speed services, such as a local area network or a DSL. Your computer is an important equipment needed. The adaptor or specialized phone is also required.
The three simple components can cover a big deal of complicated situation. Such complexity is dealt with by having time and money invested in understanding and maintaining the phone system. You may probably need to pay someone else to do it for you.
Today, VoIP phone systems provide telephone calls, where a fraction of the call routes over an IP network. Part of the call would travel over the Internet by a public or a private route. Once you are calling a regular phone, a part of the call will as well travel over the regular public telephone system or PSTN.
Your business will need to have an internal Ethernet network because of the IP network utilization for VoIP. Once you are using computers in your business, you certainly have it, so you do not have to worry. However, in case you realize that you do not have one, you can purchase an affordable one then have it easily set up.
VoIP services may allow you to call other people by using the same service; however, there are some which allow you to call anyone having a telephone number. This includes international numbers, mobile phones, local numbers, and long distance. Some VoIP services just work over your computer or a special VoIP phone, while other services let you use a traditional phone connected to the VoIP adapter.
We may consider a forecast that in the next two decades, each home will be utilizing a VoIP phone system. This will represent a considerable revolutionized communication technology. However, the experts must always consider the density of calls per second or the possible problems encumbered by traffic. Anyway, with all the innovations brought about each year, we must expect a highly transformed communication technology ahead.
Thanks for reading this article. Please link to this webpage if you want to use this article anywhere. I hope you loved what I wrote on "The VoIP phone system and our revolutionized communication technology". I would be glad to know what you think concerning it, negative or positive. Please leave a comment below and show me you're alive.
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After a pre-season shrouded in controversy and protests, Chaz Bono made his debut on Dancing with the Stars to the audience's cheers and the judges' surprising delight.
Season 13 of ABC's hit competition show kicked off with all eyes on the transgender child of Cher and the late Sonny Bono, whose presence had been loudly criticized by groups who thought him to be an inappropriate choice for the "family series." And adding to the pressure of being the first transgender contestant, was the fact Cher had admitted that he wasn't exactly the dancing type.
But when the hour was up, Chaz and partner Lacey Schwimmer had surprised the judges, who responded positively to their debut performance, a cha-cha-cha to "Dancing in the Streets."
"Chazzy Boy, you can dance!" said judge Carrie Ann Inaba.
Judge Len Goodman called Bono "Razzamataz Chaz" and praised his footwork, while Bruno Tonioli said, "So cuddly and cute. Great, great debut, Chaz. You should be proud."
The duo finished with a respectable 17 points.
But, of course, Chaz wasn't the only star to debut his fancy footwork on Monday night's show.
With ex-wife Courteney Cox and their 7-year-old daughter Coco cheering him in on, David Arquette gave a respectable 18-point debut with partner Kym Johnson, as the two danced the waltz to Queen's "Somebody to Love." And in one of the cutest moments of the night, the camera panned to a delighted Coco as Arquette gushed about his little girl: "She's just so beautiful... She's the love of my life."
But Cox wasn't the only famous face in the audience. The entire Kardashian clan showed up to support Rob Kardashian, including DWTS alum Kim Kardashian. But their presence wasn't enough to bring Rob luck, as he failed to wow the judges and received a 16 for his waltz with partner Cheryl Burke.
Ricki Lake, who has shed more than 8 inches off her figure, and her handsome young partner Derek Hough finished with a great 20 points after their waltz, while American soccer star Hope Solo and Maksim Chmerkovskiy also impressed with their 21-point opening dance.
Soap-opera star and Iraq war veteran J.R. Martinez was remarkably graceful with his partner Karina Smirnoff delivering a memorable Viennese Waltz. The couple received an impressive 22 points, tying with singer Chynna Phillips and Tony Dovolani for the night's top spot.
But it was the season's other professional athlete, Ron Artest, who drew the night's lowest score with a 14 for his first number, a cha-cha-cha with Peta Murgatroyd.
But, as always, it's the viewers' votes that really count!
Novel technique reveals both gene number and protein expression simultaneouslyPublic release date: 22-Sep-2011 [ | E-mail | Share ]
Contact: Darrell E. Ward Darrell.Ward@osumc.edu 614-293-3737 Ohio State University Medical Center
COLUMBUS, Ohio -- Researchers have discovered a method for simultaneously visualizing gene number and protein expression in individual cells. The fluorescence microscopy technique could permit a detailed analysis of the relationship between gene status and expression of the corresponding protein in cells and tissues, and bring a clearer understanding of cancer and other complex diseases, according to researchers who led the study.
The new technique is called the fluorescent in situ gene protein assay. It combines traditional fluorescent in situ hybridization (FISH) with the in situ proximity ligation assay, which is capable of resolving individual protein molecules.
"To my knowledge, this is the first technique that allows us to concurrently address gene activity and corresponding protein expression in the same cells," says co-principal investigator Dr. Arnab Chakravarti, chair and professor of radiation oncology and co-director of the Brain Tumor Program at the Ohio State University Comprehensive Cancer Center Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC - James).
"The ability to resolve gene and protein-expression changes across a tumor could help us understand what drives tumor behavior overall," Chakravarti says
The new assay is described in the August issue of the journal Neuro-Oncology.
For this study, principal investigator Dr. Markus Bredel, an associate professor at the University of Alabama Birmingham, and an adjunct associate professor radiation oncology at the OSUCCC James, along with Chakravarti and their collaborators first assayed fixed human glioblastoma tumor cells, then paraffin-embedded human glioblastoma tissue. In both cases, the researchers assayed for overexpression of a mutant form of the epidermal growth factor receptor gene, EGFRvIII, and for levels of its truncated protein in glioblastoma.
"This method has potential to perform a detailed analysis of the relationship between cancer gene status and corresponding protein expression in cells and tissues," Bredel says. "We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving cancer gene and protein patterns simultaneously on a cell-by-cell basis, which is particularly important in heterogeneous diseases such cancers."
The implications of the assay include the following:
It is particular relevant to cancer research due to the role of epigenetic and posttranscriptional regulation.
The ability to correlate gene and protein information in the same cells might increase the reliability of biomarker screens.
It might aid in therapeutic decision making when screening for only the gene or the protein yields indeterminate results.
It can be applied to gene-transfection studies that use 'knock-in' models to study the effects of gene number on protein expression.
"We believe this assay is broadly applicable to disease-oriented and cell- and molecular-biology research," Chakravarti says.
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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Novel technique reveals both gene number and protein expression simultaneouslyPublic release date: 22-Sep-2011 [ | E-mail | Share ]
Contact: Darrell E. Ward Darrell.Ward@osumc.edu 614-293-3737 Ohio State University Medical Center
COLUMBUS, Ohio -- Researchers have discovered a method for simultaneously visualizing gene number and protein expression in individual cells. The fluorescence microscopy technique could permit a detailed analysis of the relationship between gene status and expression of the corresponding protein in cells and tissues, and bring a clearer understanding of cancer and other complex diseases, according to researchers who led the study.
The new technique is called the fluorescent in situ gene protein assay. It combines traditional fluorescent in situ hybridization (FISH) with the in situ proximity ligation assay, which is capable of resolving individual protein molecules.
"To my knowledge, this is the first technique that allows us to concurrently address gene activity and corresponding protein expression in the same cells," says co-principal investigator Dr. Arnab Chakravarti, chair and professor of radiation oncology and co-director of the Brain Tumor Program at the Ohio State University Comprehensive Cancer Center Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC - James).
"The ability to resolve gene and protein-expression changes across a tumor could help us understand what drives tumor behavior overall," Chakravarti says
The new assay is described in the August issue of the journal Neuro-Oncology.
For this study, principal investigator Dr. Markus Bredel, an associate professor at the University of Alabama Birmingham, and an adjunct associate professor radiation oncology at the OSUCCC James, along with Chakravarti and their collaborators first assayed fixed human glioblastoma tumor cells, then paraffin-embedded human glioblastoma tissue. In both cases, the researchers assayed for overexpression of a mutant form of the epidermal growth factor receptor gene, EGFRvIII, and for levels of its truncated protein in glioblastoma.
"This method has potential to perform a detailed analysis of the relationship between cancer gene status and corresponding protein expression in cells and tissues," Bredel says. "We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving cancer gene and protein patterns simultaneously on a cell-by-cell basis, which is particularly important in heterogeneous diseases such cancers."
The implications of the assay include the following:
It is particular relevant to cancer research due to the role of epigenetic and posttranscriptional regulation.
The ability to correlate gene and protein information in the same cells might increase the reliability of biomarker screens.
It might aid in therapeutic decision making when screening for only the gene or the protein yields indeterminate results.
It can be applied to gene-transfection studies that use 'knock-in' models to study the effects of gene number on protein expression.
"We believe this assay is broadly applicable to disease-oriented and cell- and molecular-biology research," Chakravarti says.
###
[ | E-mail | Share ]
?
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Bionic bacteria may help fight disease and global warming Public release date: 21-Sep-2011 [ | E-mail | Share ]
Contact: Andy Hoang ahoang@salk.edu 858-453-4100 x1226 Salk Institute
Salk Institute researchers have built artificially enhanced bacteria capable of producing new kinds of synthetic chemicals
La Jolla--A strain of genetically enhanced bacteria developed by researchers at the Salk Institute for Biological Studies may pave the way for new synthetic drugs and new ways of manufacturing medicines and biofuels, according to a paper published September 18 in Nature Chemical Biology.
For the first time, the scientists were able to create bacteria capable of effectively incorporating "unnatural" amino acids - artificial additions to the 20 naturally occurring amino acids used as biological building blocks - into proteins at multiple sites. This ability may provide a powerful new tool for the study of biological processes and for engineering bacteria that produce new types of synthetic chemicals.
"This provides us with a lot more room to think about what we can do with protein synthesis," said Lei Wang, assistant professor in Salk's Chemical Biology and Proteomics Laboratory and holder of the Frederick B. Rentschler Developmental Chair. "It opens up new possibilities, from creating drugs that last longer in the blood stream to manufacturing chemicals in a more environmentally friendly manner."
In 2001, Wang and his colleagues were the first to create bacteria that incorporated unnatural amino acids (Uaas) into proteins, and, in 2007, they first used the technique in mammalian cells. They did this by creating an "expanded genetic code," overriding the genetic code of the cells and instructing them to use the artificial amino acids in the construction of proteins.
The addition of Uaas changes the chemical properties of proteins, promising new ways to use proteins in research, drug development and chemical manufacturing.
For instance, Wang and his colleagues have inserted Uaas that will glow under a microscope when exposed to certain colors of light. Because proteins serve as the basis for a wide range of cellular functions, the ability to visualize this machinery operating in live cells and in real time helps scientists decipher a wide range of biological mechanisms, including those involved in the development of disease and aging.
Genetically modified bacteria are already used for producing medicines, such as synthetic insulin, which has largely replaced the use of animal pancreases in the manufacture of drugs used by diabetics to regulate their blood sugar levels.
To date, such recombinant DNA technology has used only natural amino acids, which limits the possible functions of the resulting protein products. The ability to insert Uaas could dramatically expand the possible uses of such technology, but one major barrier has limited the use of Uaas: only a single Uaa at a time could be incorporated into a protein.
To insert the instructions for including a Uaa in a bacterium's genetic code, Wang and his colleagues exploited stop codons, special sequences of code in a protein's genetic blueprint. During protein production, stop codons tell the cellular machinery to stop adding amino acids to the sequence that forms backbone of the protein's structure.
In 2001, Wang and his colleagues modified the genetic sequence of the bacteria Escherichia coli to selectively include a stop codon and introduced engineered molecules inside the bacteria, which surgically insert a Uaa at the stop codon. This trained the bacteria to produce proteins with the Uaa incorporated in their backbone.
The problem was that another biological actor, a protein known as release factor 1 (RF1), would stop the production of a Uaa-containing protein too early. Although scientists could insert stop codons for Uaas at multiple places along genetic sequence, the release factor would cut the protein off at the first stop codon, preventing production of long proteins containing multiple Uaas.
"To really make use of this technology, you want to be able to engineer proteins that contain unnatural amino acids at multiple sites, and to produce them in high efficiency," Wang said. "It was really promising, but, until now, really impractical."
In their new paper, the Salk researchers and their collaborators at the University of California, San Diego described how they got around this limitation. Since RF1 hindered production of long Uaa-containing proteins, the scientists removed the gene that produces RF1. Then, because E. Coli dies when the RF1 gene is deleted, they altered production of an alternative actor, release factor 2 (RF2), so that it could rescue the engineered bacterium.
The result was a strain of bacteria capable of efficiently producing proteins containing Uaas at multiple places. These synthetic molecules hold promise for the development of drugs with biological functions far beyond what is possible with proteins that include only naturally occurring amino acids. They may also serve as the basis for manufacturing everything from industrial solvents to biofuels, possibly helping to address the economic and environmental concerns associated with petroleum-based manufacturing and transportation.
"This is the first time we've been able to produce a viable strain of bacteria capable of this," Wang said. "We still have a ways to go, but this makes the possibility of using unnatural amino acids in biological engineering far closer to being reality."
###
The research was funded by the Beckman Young Investigator Program, the California Institute for Regenerative Medicine, the March of Dimes Foundation, the Mary K. Chapman Foundation, the National Institutes of Health, the National Science Foundation, the Pioneer Fellowship, the Ray Thomas Edwards Foundation and the Searle Scholar Program.
[ | E-mail | Share ]
?
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
Bionic bacteria may help fight disease and global warming Public release date: 21-Sep-2011 [ | E-mail | Share ]
Contact: Andy Hoang ahoang@salk.edu 858-453-4100 x1226 Salk Institute
Salk Institute researchers have built artificially enhanced bacteria capable of producing new kinds of synthetic chemicals
La Jolla--A strain of genetically enhanced bacteria developed by researchers at the Salk Institute for Biological Studies may pave the way for new synthetic drugs and new ways of manufacturing medicines and biofuels, according to a paper published September 18 in Nature Chemical Biology.
For the first time, the scientists were able to create bacteria capable of effectively incorporating "unnatural" amino acids - artificial additions to the 20 naturally occurring amino acids used as biological building blocks - into proteins at multiple sites. This ability may provide a powerful new tool for the study of biological processes and for engineering bacteria that produce new types of synthetic chemicals.
"This provides us with a lot more room to think about what we can do with protein synthesis," said Lei Wang, assistant professor in Salk's Chemical Biology and Proteomics Laboratory and holder of the Frederick B. Rentschler Developmental Chair. "It opens up new possibilities, from creating drugs that last longer in the blood stream to manufacturing chemicals in a more environmentally friendly manner."
In 2001, Wang and his colleagues were the first to create bacteria that incorporated unnatural amino acids (Uaas) into proteins, and, in 2007, they first used the technique in mammalian cells. They did this by creating an "expanded genetic code," overriding the genetic code of the cells and instructing them to use the artificial amino acids in the construction of proteins.
The addition of Uaas changes the chemical properties of proteins, promising new ways to use proteins in research, drug development and chemical manufacturing.
For instance, Wang and his colleagues have inserted Uaas that will glow under a microscope when exposed to certain colors of light. Because proteins serve as the basis for a wide range of cellular functions, the ability to visualize this machinery operating in live cells and in real time helps scientists decipher a wide range of biological mechanisms, including those involved in the development of disease and aging.
Genetically modified bacteria are already used for producing medicines, such as synthetic insulin, which has largely replaced the use of animal pancreases in the manufacture of drugs used by diabetics to regulate their blood sugar levels.
To date, such recombinant DNA technology has used only natural amino acids, which limits the possible functions of the resulting protein products. The ability to insert Uaas could dramatically expand the possible uses of such technology, but one major barrier has limited the use of Uaas: only a single Uaa at a time could be incorporated into a protein.
To insert the instructions for including a Uaa in a bacterium's genetic code, Wang and his colleagues exploited stop codons, special sequences of code in a protein's genetic blueprint. During protein production, stop codons tell the cellular machinery to stop adding amino acids to the sequence that forms backbone of the protein's structure.
In 2001, Wang and his colleagues modified the genetic sequence of the bacteria Escherichia coli to selectively include a stop codon and introduced engineered molecules inside the bacteria, which surgically insert a Uaa at the stop codon. This trained the bacteria to produce proteins with the Uaa incorporated in their backbone.
The problem was that another biological actor, a protein known as release factor 1 (RF1), would stop the production of a Uaa-containing protein too early. Although scientists could insert stop codons for Uaas at multiple places along genetic sequence, the release factor would cut the protein off at the first stop codon, preventing production of long proteins containing multiple Uaas.
"To really make use of this technology, you want to be able to engineer proteins that contain unnatural amino acids at multiple sites, and to produce them in high efficiency," Wang said. "It was really promising, but, until now, really impractical."
In their new paper, the Salk researchers and their collaborators at the University of California, San Diego described how they got around this limitation. Since RF1 hindered production of long Uaa-containing proteins, the scientists removed the gene that produces RF1. Then, because E. Coli dies when the RF1 gene is deleted, they altered production of an alternative actor, release factor 2 (RF2), so that it could rescue the engineered bacterium.
The result was a strain of bacteria capable of efficiently producing proteins containing Uaas at multiple places. These synthetic molecules hold promise for the development of drugs with biological functions far beyond what is possible with proteins that include only naturally occurring amino acids. They may also serve as the basis for manufacturing everything from industrial solvents to biofuels, possibly helping to address the economic and environmental concerns associated with petroleum-based manufacturing and transportation.
"This is the first time we've been able to produce a viable strain of bacteria capable of this," Wang said. "We still have a ways to go, but this makes the possibility of using unnatural amino acids in biological engineering far closer to being reality."
###
The research was funded by the Beckman Young Investigator Program, the California Institute for Regenerative Medicine, the March of Dimes Foundation, the Mary K. Chapman Foundation, the National Institutes of Health, the National Science Foundation, the Pioneer Fellowship, the Ray Thomas Edwards Foundation and the Searle Scholar Program.
[ | E-mail | Share ]
?
AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.
So you are Internet Marketing efforts are making benefits, but not Huge outcomes. You do some study and understand that video is generating wonderful benefits for marketers. You experiment and generate some ?how to? videos but now how do you share them with your clients?
You can upload and share them via YouTube, Vimeo, or any of the other video streaming hosting suppliers but there is a catch. The catch is that this ?free? internet hosting comes with many distractions such as relevant video clips that can send your clients away from your website.
As a intelligent World wide web marketer, you know this is the previous thing you want. And now you understand you need a business video hosting supplier!
Oh sure, you could be able to publish your films on your world wide web host internet hosting account but until you have a quality organization world wide web host hosting account, you will quickly exceed your storage and bandwidth limits. At a bare minimum, you will have to improve your account and devote a lot more cash each and every month. At worst, you could even be banned and uncover oneself out of organization whilst you work to get your account restored.
So here is a list of things to contemplate when searching for a industrial video streaming hosting resolution:
Dependability- If your video streaming hosting organization goes down, youre out of organization. Thus dependability and uptime are vital to your enterprise! Make sure your video hosting organization has enough video streaming hosting servers for the amount of buyers it supports.
Technical Assistance ? When your commercial video clip hosting supplier isnt really delivering your video streams, you require support immediately, not tomorrow morning.
Storage Space ? Video clip information can require huge quantities of room. Make confident your business video internet hosting supplier permits you ample storage area for your video files.
Bandwidth ? If your video streaming hosting firm cannot provide ample bandwidth to provide your movies without having pauses, hiccups, or pixelation, your buyers will not view.
As you can see, it is imperative you find a reputable industrial video internet hosting organization.
Drake tax preparation software is most widely used and trusted tax preparation software among majority of accounting professionals. Drake software stands out from other tax software in terms of overall customer satisfaction, reliability, customer support and easy to use features. More-over majority of small and medium business firms favors using tax software like drake for trouble free, accurate, timely, smooth tax filing and preparation.
Aid to your businesses
A number of small and medium businesses are cropping up every day all over the world. Majority of them are not able to survive and are shut down with in first 2, 3 years. Primary reason for their failure can be given as diversion from the core business goals and allocating their time and resources to day to day operational activities. These back office tasks brings down the profitability curve of the businesses as they gulp down majority of resources.
So tax return filing and preparation consumes a considerable AMOUNT OF MONEY and resources of an organization. Easy way out of these scenarios is hire professionals like CPAs or using hosted tax software like drake. Even CPAs uses tax software generally .So ultimate solution is a good tax preparation software.
Tax software is acclaimed for data accuracy, faster tax return preparation and integration with a large number of add-ons for efficiency and comprehensiveness of forms supported by the application. This software saves you from cumbersome calculations and paper work. ALL you have to do is provide the needed information. This software will then guide you through the entire process and ensure an accurate and error free tax return preparation.
Drake software gives guaranteed accuracy in tax preparation, quick time tax filing. Process of tax preparation is further eased and made efficient by cloud hosting technologies. Tax software is made available as hosted applications to customers by many service providers. These services are definitely better than the desktop deployment of tax software. Benefits of using hosted tax software in cloud environment can be summed up as:-
Anytime access to your data:-Tax software hosting allows a user to have anytime and anywhere access to hi or her tax data. So user has greater control on their tax data management from anywhere. This is prominent feature of cloud hosting services.
Cost reduction: ? Major benefit of application hosting is reduction in cost. This reduction in cost can be attributed to factors like refraining from investment in infrastructure (procuring, maintaining servers), data backup and security.
Expertise of service providers :- User does not have to spend time in understanding the tools .All of service provider have well trained staff which have expertise in all tax computation tools .This ensures faster response and accurate tax preparation .
Available ADD-ONS:- Various ADD-ONS for tax software are easily available in market which enhances functionality of tax software and make the more industry specific. These utilities make tax software more flexible. Document esort, client manager are examples of type add-ons present for tax software in market.
Above stated benefits justifies growing popularity of hosted tax software like Drake. By Opting for hosted tax software small and medium business can definitely save their resources and concentrate more on their core business needs. Effects of using hosted tax software are definitely positive and can be felt across various domains of small and medium business organization.
Many business man want to know.. Me and my friend likebuyonme shopandEbay. If you also want to choose a legit store without online scam, the following factors are something you should consider:
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- Safe and convenient payment method
- Helpful and trustworthy CS team
- With good products quality
- Shipping cost is not too high
- Legit website without scam
- Various categories for shopping
- Cheap price for items
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buyonme shop shipping time is no more than half a month. Most package are no more than 2 weeks. The longest time I have waited is about 21 days. Shipping time is not very important to me if I finally receive the item. Pet Care from Buyonme are in good quality with cheap price. Why not give a try?
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I have purchased the following items.
Quality Adjustable Shoulder Strap Dog Leash (1.4m Cable)
Cute Walking Shoes for Pets/Dogs ? Color Assorted (Size XL/2-Pair)
Check Shirt with Headpiece Pet Clothing (Color Assorted)
Stainless Steel Large Dog De-Shedding Tool ? Yellow+Black
L ? Super Man Dog Costume Pet Clothes Super-Doggy
Classic Style Jacket for Pets (Size-L)
Flea Collar for Dogs (2-Pack)
Chinese Style Tang Suit Dog Apparel Pet Clothes ? Hot Pink (Size-S/10)
Cute Christmas Style Pet Cloak Dog Apparel with Hat ? Color Assorted (Size M)
3.5-inch Hair Comb for Pets (Cats & Dogs)
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The store also sells various kinds of things, Besides above products.
Such as: USB Hubs & Switches, Other Games, LED Clothing, Keyboards, Android Tablet, Pet Care, Other Occasion, Adapter & Dongle, Cell Phone, Women?s Clothing, Cables, Mouse, Rings, cell phones, Cooler & Fans, Microphones, Networking, Laptops, USB Gadgets, Auto Parts Car Acce, DV & Recorders, Sony PSP, Rare Earth/RE Magnets, Occasion & Holiday, Others, DIY Parts, April Fool?s Day, Sports products, PC & Accessories, Headsets, Apparels, Knives & Tools, Cell Phone Accessories, Bags, Earrings, iPad & iPhone, Kitchen Gadgets, Others, Home & Office, Converters & Plug,. I think you could find your favortie items among them.
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Other legit online stores I used to shopping at:
buyincoins ? Cool gadgets, cheap price
gmarket.co.kr ? Korean site with a wide choice
tinydeal ? Cool gadgets, free shipping
dinodirect ? hong kong based
focalprice ? the price is little higher
buyonme.com ? cheap price, free shipping
brandsdragon.com ? funny gadgets, the price is little higher
spider-foot.com ? covers,cases for mobile, notebook
dealextreme ? long history well known online store
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You buy thing from the Internet, you never see the face of the seller, you just pay and wait for your package. It could not be imagined in 10 years ago. But it is reality now. Isn?t it wonderful?
Related posts:
How To Purchase China Pet Care products and why I choose BuyOnMe store?
Where Can I Buy Wholesale Pet Care products and why I choose buyonme store?
Can I Find China Mouse products and why I choose BuyOnMe shop?
How To Find Cheapest Cameras & Acce products and why I choose BuyOnMe shop?
Where To Purchase Cheapest LED Clothing products and why I choose BuyOnMe gadgets shop?
Someone Could Tell Me Where To Purchase Made In China Movie Souvenirs products and why I choose BuyOnMe eshop?
Who Can Tell Me How To Purchase Wholesale Flashlights products and why I choose BuyOnMe shop?
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Public release date: 22-Sep-2011
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